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Chapters
- 00:05Title
- 01:16Seeding Endothelial Cells (RBMECs)
- 03:31Oxygen and Glucose Deprivation-Reoxygenation In Vitro Model
- 04:40Immunofluorescence Localization and f-actin Labeling with Rhodamine Phalloidin
- 07:29An Oxygen-Glucose Deprivation and Reoxygenation (OGD-R) Experiment
- 08:22Conclusion
Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.
