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Chapters
- 00:05Title
- 01:41Embryo and Gut Dissection
- 03:38Fixation of GI Tracts
- 04:34Immunostaining Protocol
- 06:11Results: Representative Images of Cholinergic Neurons in the Distal Small Intestine and Colon
- 07:19Conclusion
The enteric nervous system is formed by neural crest cells that proliferate, migrate and colonize the gut. Neural crest cells differentiate into neurons with markers specific for their neurotransmitter phenotype. This protocol describes a technique for dissecting, fixing and immunostaining of the murine embryonic gastrointestinal tract to visualize enteric nervous system neurotransmitter expression.
