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Chapters
- 00:05Title
- 02:39mRNA Synthesis
- 03:55Microinjection of Synthesized RNA and Stimulation of Oocyte Maturation
- 05:15Germinal Vesicle Breakdown and Western Blot Analysis
- 06:21Polyadenylation Assay
- 08:08Results: Xenopus Oocyte Translation Analysis on Maturation
- 09:43Conclusion
Protein synthesis control occurs mainly at the translation initiation step, deficiencies in which are linked to diverse disorders. To better understand their etiology, we described here a protocol using Xenopus laevis oocytes assessing the translation of mos transcript in the presence of a mutant of translation initiation factor eIF4G1.
