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Chapters
- 00:05Title
- 01:55Setting Up for Confocal Scanning Laser Ophthalmoscopy (cSLO)
- 02:57In Vivo Imaging of Retinal Microglia by cSLO
- 06:26Live Image Processing and Microglial Cell Segmentation
- 08:19Live Image Analysis and Microglial Morphometry
- 10:22Results: cSLO Imaging of Retinal Microglia Cells
- 11:41Conclusion
Microglia activation and microgliosis are key responses to chronic neurodegeneration. Here, we present methods for in vivo, long-term visualization of retinal CX3CR1-GFP+ microglial cells by confocal ophthalmoscopy, and for threshold and morphometric analyses to identify and quantify their activation. We monitor microglial changes during early stages of age-related glaucoma.
