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Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
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Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

DOI:
10.3791/52732-v

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08:26 min

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May 14, 2015

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,
1Division of Genetics and Development,Toronto Western Research Institute and Krembil Neuroscience Center, 2Department of Surgery, Division of Neurosurgery,Toronto Western Hospital and University of Toronto

Chapters

  • 00:05Title
  • 01:29Harvesting and Dissection of Adult Rat Periventricular Spinal Cord Tissue
  • 03:43Isolation and Culturing of Adult Rat Neural Stem/Progenitor Cells from the Spinal Cord
  • 06:51Results: Isolated Neurospheres Express Ki67 and Nestin by Passage 3
  • 07:50Conclusion

Summary

자동 번역

자동 번역

The adult mammalian spinal cord contains neural stem/progenitor cells (NSPCs) that can be isolated and expanded in culture. This protocol describes the harvesting, isolation, culture, and passaging of NSPCs generated from the periventricular region of the adult spinal cord from the rat and from human organ transplant donors.

Tags

Keywords: Neural Stem CellsNeural Progenitor CellsSpinal CordAdultRatHumanNeurosphereProliferationDifferentiationNeuronAstrocyteOligodendrocyteEpidermal Growth FactorFibroblast Growth FactorHeparinNeural Stem Cell CultureNeural Stem Cell Transplantation

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