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Chapters
- 00:05Title
- 03:31Larvae Collection and Dissection
- 05:58Quantification
- 07:30Hemocyte Disturbance Assay
- 09:03Results: Transient Changes in Circulating and Resident Hemocyte Populations
- 09:54Conclusion
Drosophila blood cells, or hemocytes, cycle between resident sites and circulation. In the larva, resident (sessile) hemocytes localize to inductive microenvironments, the Hematopoietic Pockets, while circulating hemocytes move freely in the hemolymph. The goal of this protocol is the standardized isolation and quantification of these two, behaviorally distinct but interchanging, hemocyte populations.
