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Chapters
- 00:05Title
- 02:20Preparation of Rat Aorta, Dye Loading, and Incubation
- 03:26Laser Scanning Two Photon Microscopy Protocol
- 04:39Image Processing and Calculations
- 05:55Results: Intracellular Calcium in the Rat Aorta
- 07:08Conclusion
Vascular cell functiondepends on activity of intracellular messengers. Described here is an ex vivo two photon imaging method that allows the measurement of intracellular calcium and nitric oxide levels in response to physiological and pharmacological stimuli in individual endothelial and smooth muscle cells of an isolated aorta.
