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Live cell imaging tijdens Mechanical Stretch
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Live Cell Imaging during Mechanical Stretch
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Live cell imaging tijdens Mechanical Stretch

DOI:
10.3791/52737-v

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07:42 min

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August 19, 2015

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, , , , ,
1Department of Physiology,University of Tennessee Health Science Center, 2Department of Biomedical Engineering and Imaging,University of Tennessee Health Science Center, 3Department of Biomedical Engineering,University of Memphis, 4Department of Engineering Technology,University of Memphis

Chapters

  • 00:05Title
  • 01:29Application of Stretch on Mouse Lung Epithelial Cell Line
  • 03:22Application of Stretch and Atomic Force Microscopy
  • 05:15Nano-indentation of Stretched Cells
  • 05:44Results: Elastic Modulus Mapping of Mouse Lung Epithelial Cells Under Strain
  • 06:57Conclusion

Summary

자동 번역

자동 번역

A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.

Tags

Keywords: Live Cell ImagingMechanical StretchCell ActuatorConfocal MicroscopyAtomic Force Microscopy (AFM)Mitochondrial SuperoxideAlveolar Epithelial CellsLung InjuryNano-indentation

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