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Misura Stress ossidativo Resistenza<em> Caenorhabditis elegans</em> In 96 pozzetti micropiastre
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Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates
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Misura Stress ossidativo Resistenza<em> Caenorhabditis elegans</em> In 96 pozzetti micropiastre

DOI:
10.3791/52746-v

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08:10 min

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May 09, 2015

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,
1Goodman Cancer Research Center,McGill University, 2Department of Biochemistry,McGill University

Chapters

  • 00:05Title
  • 00:57Preparation of Media for C. elegans Growth
  • 02:15Preparation for Gene Downregulation Using RNAi
  • 03:50Preparation of Age-synchronized L4 Population
  • 04:45Performing the Oxidative Stress Resistance Assay
  • 06:43Results: flcn-1(ok975) Mutants are More Resistant than Wild-type C. elegans to Oxidative Stress
  • 07:38Conclusion

Summary

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자동 번역

C. elegans is an attractive model organism to study signal transduction pathways involved in oxidative stress resistance. Here we provide a protocol to measure oxidative stress resistance of C. elegans animals in liquid phase, using several oxidizing agents in 96 well plates.

Tags

Caenorhabditis ElegansOxidative StressReactive Oxygen Species96-well Microtiter PlateParaquatH2O2L4/young AdultGenetic ScreensOxidative Stress Signal Transduction Pathways

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