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Chapters
- 00:05Title
- 01:19Genomic DNA Quality Control and Amplicon Library Preparation
- 03:23Indexing and PCR Amplification
- 04:37PCR Purification and Bead-based Normalization
- 06:33Sequencing and Data Analysis
- 07:19Results: Mutations Detected in Solid and Liquid Tumors Using Next Generation Sequencing
- 09:06Conclusion
This manuscript describes clinical protocols for two next-generation sequencing panels. One panel interrogates hematologic malignancies while the other panel targets genes commonly mutated in solid tumors. Molecular classification of driver mutations in human malignancies offers valuable prognostic and predictive information.
