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Misura High Throughput di extracellulare DNA di uscita e quantitativa NET Formazione in neutrofili umani<em> In Vitro</em
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
DOI:

11:03 min

June 18, 2016

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Chapters

  • 00:05Title
  • 00:49Isolation of Neutrophils from Peripheral Human Blood
  • 02:17Measurement of Kinetics of Extracellular DNA Release Using Microplate Fluorimeter
  • 04:22Quantitation of NET Formation by MPO-DNA and HNE-DNA ELISA Assays
  • 08:22Results: MPO-DNA and HNE-DNA ELISA Assay Results
  • 10:25Conclusion

Summary

자동 번역

High throughput assays are presented that in combination provide excellent tools to quantitate NET release from human neutrophils.

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