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Chapters
- 00:05Title
- 01:30Embryo/larva Preparation
- 02:42Metronidazole (Mtz) Treatment and Biliary-driven Liver Regeneration Analysis
- 06:07Results: Representative Liver Ablation and Regeneration Analysis
- 07:24Conclusion
To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.
