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Ikke-invasiv billeddannelse af Innate immunrespons i en zebrafisk Larve Model af<em> Streptococcus iniae</em> Infektion
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Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection

Ikke-invasiv billeddannelse af Innate immunrespons i en zebrafisk Larve Model af<em> Streptococcus iniae</em> Infektion

DOI:
10.3791/52788-v

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11:16 min

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April 21, 2015

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,
1Microbiology Doctoral Training Program,University of Wisconsin-Madison, 2Department of Medical Microbiology and Immunology,University of Wisconsin-Madison, 3Department of Pediatrics,University of Wisconsin-Madison

Chapters

  • 00:05Title
  • 01:23Preparation of Larval Injection Dishes
  • 02:31Otic Vesicle Injection of Streptococcus iniae in Three Day Old Larvae
  • 06:19Preparation of Larvae for Live Imaging
  • 07:34Photoconversion of Dendra2-labeled Leukocytes at the Otic Vesicle
  • 08:56Results: Streptococcus iniae Infection in the Zebrafish Larval Otic Vesicle
  • 10:28Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol for the generation and imaging of a localized bacterial infection in the zebrafish otic vesicle.

Tags

Keywords: ZebrafishStreptococcus IniaeInnate ImmunityNon-invasive ImagingNeutrophilsMacrophagesOtic VesicleLocalized InfectionPhotolabeling

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