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蛋白质直接送货到哺乳动物细胞利用细胞渗透半胱氨酸<sub> 2</sub> -His<sub> 2</sub>锌指域

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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys₂-His₂ Zinc-finger Domains

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蛋白质直接送货到哺乳动物细胞利用细胞渗透半胱氨酸<sub> 2</sub> -His<sub> 2</sub>锌指域

DOI:

10.3791/52814-v

•

11:24 min

•

March 25, 2015

•

Thomas Gaj*¹, Jia Liu*^1,2

¹Departments of Chemistry and Cell and Molecular Biology,The Scripps Research Institute, ²Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University

Chapters

00:05Title
01:23Cloning Emerald GFP into an Expression Vector Containing Two-finger ZiF Domains
04:22Purification of the ZiF-EmGFP Fusion Protein
07:17Protein Transduction of HeLa Cells
10:07Results: Fusion of 2F-ZiF Domains to EmGFP Enables Delivery of EmGFP to HeLa Cells
10:57Conclusion

Summary

자동 번역

锌指结构域是本质细胞渗透性和能够介导蛋白输送到广泛的哺乳动物细胞类型的。这里，一个详细的一步一步的协议用于实现锌指技术为细胞内蛋白输送呈现。

Tags

Keywords: Zinc-finger Protein Delivery Cell-permeable Cys2-His2 Genome Engineering Intracellular Delivery Cell Membrane Protein Transduction Enzyme Activity Cytosolic Delivery Mammalian Cells

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