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成人人成纤维细胞的推导和他们直接转换成可扩展的神经祖细胞

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Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

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成人人成纤维细胞的推导和他们直接转换成可扩展的神经祖细胞

DOI:

10.3791/52831-v

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13:58 min

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July 29, 2015

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Sandra Meyer², Philipp Wörsdörfer, Katharina Günther, Marc Thier³, Frank Edenhofer²

¹Institute of Anatomy and Cell Biology,University of Würzburg, ²Institute of Reconstructive Neurobiology,University of Bonn, ³German Cancer Research Center, Heidelberg

Chapters

00:05Title
02:21Punch Biopsy
06:12Infection of Human Fibroblasts with Sendai Virus and Transdifferentiation
08:47Isolation of Putative Induced Neural Progenitor Cells (iNPC) Colonies
10:15Differentiation of iNPCs
12:04Results: Reprogramming of Fibroblasts into Neural Precursors and their Cellular Validation
13:20Conclusion

Summary

자동 번역

诱导多能干细胞的产生提供了用于自体移植的推导引人入胜的前景。然而，通过多能状态和艰苦的再分化仍然进展阻碍临床翻译。在这里，我们描述了成年人成纤维细胞的衍生和其直接转化为诱导神经祖细胞和随后的分化成神经谱系。

Tags

Fibroblasts Induced Pluripotent Stem Cells IPSCs Neural Progenitor Cells INPCs Direct Conversion Autologous Cell Replacement Disease Modeling Drug Screening Oct4 Sox2 Klf4 C-Myc Nestin TUJ1 GFAP

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