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方法来提高高分辨率的敏感性疟疾熔单核苷酸多态性基因分型

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

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方法来提高高分辨率的敏感性疟疾熔单核苷酸多态性基因分型

DOI:

10.3791/52839-v

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10:27 min

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November 10, 2015

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Rachel Daniels², Elizabeth J. Hamilton, Katelyn Durfee, Daouda Ndiaye, Dyann F. Wirth⁵, Daniel L. Hartl, Sarah K. Volkman⁴

¹Department of Organismic and Evolutionary Biology,Harvard University, ²Department of Immunology and Infectious Diseases,Harvard T.H. Chan School of Public Health, ³Faculty of Medicine and Pharmacy,Cheikh Anta Diop University, ⁴School of Nursing and Health Sciences,Simmons College, ⁵Institute of Infectious Diseases,Broad Institute

Chapters

00:05Title
01:58Template Preparation
03:46PCR Amplification
05:37HRM Analysis
08:26Results: Blocked-probe-based and MAAB Increases HRM Genotyping Sensitivity
09:46Conclusion

Summary

자동 번역

虽然高分辨率熔解分析提供了一个异质群体的单核苷酸多态性区分的能力，突变等位基因扩增偏置可提高其检测存在于样品中相对较低的百分比等位基因的能力。本协议描述的改进，提高了高分辨率熔解分析的灵敏度。

Tags

Keywords: Malaria Plasmodium Single-nucleotide Polymorphisms (SNPs)Drug Resistance Population Surveillance High-resolution Melting (HRM) Analysis Polygenomic Infections PCR Amplification Minor Allele Genotyping Sensitivity

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