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Chapters
- 00:05Title
- 01:05Seeding of Epithelial Cell Lines
- 02:14Transfect Cells with siRNA
- 03:33Lyse Cells for Immunoblotting Analysis
- 04:22BRCA2 Immunoblotting Analysis
- 07:12Results: Effects of BRCA2 Silencing on Gene Function
- 08:42Conclusion
Gene silencing by siRNA represents a convenient experimental strategy to analyze BRCA2-dependent biological functions with immediate implications to better understand cancer biology. A method to efficiently silence BRCA2, along with the experimental procedure to detect and quantify changes in BRCA2 protein expression by immunoblotting in human cell lines, is presented.
