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Chapters
- 00:05Title
- 01:01Harvesting the Heart
- 03:58Langendorff Perfusion and Ischemia Reperfusion Injury
- 06:31Analysis of Ischemia Reperfusion Injured Hearts
- 07:32Conclusion
The isolated rat heart is an enduring model for ischemia reperfusion injury. Here, we describe the process of harvesting the beating heart from a rat via in situ aortic cannulation, Langendorff perfusion of the heart, simulated ischemia-reperfusion injury, and infarct staining to confirm the extent of ischemic insult.
