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Readout גורף פשוט של דיגיטלי מבחני כימות חומצות גרעין
JoVE 신문
생물학
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JoVE 신문 생물학
Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
DOI:

06:55 min

September 24, 2015

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Chapters

  • 00:05Title
  • 01:03Preparing Reaction Mix for Bulk Droplet Readout
  • 02:47Droplet Formation
  • 04:05Isothermal Amplification, Data Acquisition and Analysis
  • 05:12Results: Quantification of Nucleic Acids with a Simplified Readout
  • 06:33Conclusion

Summary

자동 번역

We describe an endpoint digital assay for quantifying nucleic acids with a simplified (analog) readout. We measure bulk fluorescence of droplet-based digital assays using a standard qPCR machine rather than specialized instrumentation and confirm our results by microscopy.

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