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Chapters
- 00:05Title
- 01:34Prepare Yeast Genomic DNA
- 03:15Prepare and Disperse qPCR Master Mix Template DNA and Primers
- 05:05Real Time PCR and Data Analysis
- 05:50Results: qPCR Results with WT and Synthetic Yeast gDNA and Primers
- 06:39Conclusion
Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.
