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Chapters
- 00:05Title
- 01:17Hydrophillic Glass Coverslips Preparation
- 03:00Hydrophobic Glass Coverslips Preparation
- 03:493D Silicone Mold Assembly
- 05:43Creation of Collagen Mixture and 3D Matrix
- 07:53Results: Chemoattractants Used in Planar Gradient Diffusion System Directs Cell Movement through Collagen Matrix
- 09:03Conclusion
Cell migration is an important part of human development and life. In order to understand the mechanisms that can alter cell migration, we present a planar gradient diffusion system to investigate chemotaxis in a 3D collagen matrix, which allows one to overcome modern diffusion chamber limitations of existing assays.
