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Chapters
- 00:05Title
- 01:33Preparation of Durotaxis Chambers
- 03:56Cell Plating
- 05:33Live-cell Imaging
- 06:48Data Analysis
- 08:17Results: The Focal Adhesion Protein, cdGAP, is Required for Durotaxis
- 09:24Conclusion
Many mammalian cells preferentially migrate towards a more rigid matrix or substrate through durotaxis. The goal of this protocol is to provide a simple in vitro system that can be used to study and manipulate cell durotaxis behaviors by incorporating polydimethylsiloxane (PDMS) substrates of defined rigidity, interfacing with glass coverslips.
