Journal
/
생물학
/
Produktion von Nurr-1 spezifischen polyklonalen Antikörper Frei von Kreuzreaktivität gegen seine Close Homologe, Nor1 und Nur77
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77
Please note that all translations are automatically generated. Click here for the English version.

Produktion von Nurr-1 spezifischen polyklonalen Antikörper Frei von Kreuzreaktivität gegen seine Close Homologe, Nor1 und Nur77

DOI:
10.3791/52963-v

•

12:30 min

•

August 17, 2015

•

, , , , ,
1Molecular Neurobiology Laboratory, Psychiatry,McLean Hospital, 2Department of Biochemistry, College of Medicine,Konyang University

Chapters

  • 00:05Title
  • 01:31Coupling of LBD Proteins to AminoLink Coupling Resin
  • 04:11Blocking Remaining Sites
  • 05:43Purification of Nurr1 Specific Antibodies
  • 07:21ELISA-based Analysis of Purified Anti-Nurr1 Antibody
  • 10:04Results: Pre-adsorption Against Cross-reactive Antigens Increases Specificity of Anti-Nurr1 Polyclonal Antibodies
  • 11:56Conclusion

Summary

자동 번역

자동 번역

Here, we selectively target antibodies against a specific member of a highly conserved family of proteins by immunizing animals with their most divergent regions followed by removing cross reactive antibodies by pre-adsorption.

Tags

Nurr1NR4A2Nur77NR4A1NOR1NR4A3Polyclonal AntibodiesCross-reactivityDNA Binding DomainLigand-binding DomainParkinson’s DiseaseSchizophreniaManic DepressionHippocampusLearningMemoryAffinity ChromatographyProtein APre-adsorption

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/52963?article_type=v

Operanten Lernens von Drosophila an der Torque Meter
check_url/kr/52963?article_type=v

Technische Demonstration von Whole Genome Array Comparative Genomic Hybridization
check_url/kr/52963?article_type=v

In vivo Ca 2 + - Imaging von Pilzkörper Neuronen während olfaktorischen Lernen in der Honigbiene
check_url/kr/52963?article_type=v

Autonom Bioluminescent Säugerzellen für die kontinuierliche und Echtzeit-Überwachung von Zytotoxizität
check_url/kr/52963?article_type=v

Design und Analyse des Temperaturpräferenzverhaltens und seines circadianen Rhythmus in Drosophila
check_url/kr/52963?article_type=v

Rückstands spezifische Einbau nichtkanonischer Aminosäuren in Modellproteine ​​eine Verwendung<em> Escherichia coli</em> Zellfreie Transkription-Translation-System
check_url/kr/52963?article_type=v

Verwendung von Anti-Phospho-girdin Antikörper gegen Darm Büschel Zellen frei schwebenden Maus Jejunum Cryosections visualisieren
check_url/kr/52963?article_type=v

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
check_url/kr/52963?article_type=v

Eine einfache Trockenschnittmethode zum Erhalt von Vollkornharzund und ihren Anwendungen
check_url/kr/52963?article_type=v

Schnelle, kostengünstige und unkomplizierte Herstellung von bakteriellem zellfreiem Lysat

Read Article