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Chapters
- 00:05Title
- 00:54Preparing Aortic Segments
- 03:14Seeding Aortic Segments on a Matrix
- 04:48Passaging the Endothelial Cells
- 06:41Aorta-derived Endothelial Cell Characterization
- 07:48Conclusion
The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.
