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생물학

Implementieren der Patch-Clamp und Live-Fluoreszenzmikroskopie, um funktionelle Eigenschaften von frisch isolierten PKD Epithel-Monitor

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Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

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Implementieren der Patch-Clamp und Live-Fluoreszenzmikroskopie, um funktionelle Eigenschaften von frisch isolierten PKD Epithel-Monitor

DOI:

10.3791/53035-v

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08:46 min

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September 01, 2015

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Tengis S. Pavlov, Daria V. Ilatovskaya, Oleg Palygin, Vladislav Levchenko, Oleh Pochynyuk, Alexander Staruschenko

¹Department of Physiology,Medical College of Wisconsin, ²Department of Integrative Biology & Pharmacology,University of Texas Health Science Center at Houston

Chapters

00:05Title
02:14Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments
05:02Single Channel Patch-clamp Electrophysiology
05:32Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells
06:55Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats
08:08Conclusion

Summary

자동 번역

Ionenkanäle in renale tubuläre Epithel ausgedrückt spielen eine bedeutende Rolle in der Pathologie der polyzystischen Nierenerkrankung. Hier beschreiben wir experimentelle Protokolle verwendet, um Patch-Clamp-Analyse und intrazellulären Calcium-Pegelmessungen in Zystenepithel frisch aus Nagetier Nieren getrennt durchzuführen.