Journal
/
생물학
/
Implementering Patch Clamp og Live Fluorescensmikroskopi til Monitor funksjonelle egenskaper nyisolerte PKD Epitel
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementering Patch Clamp og Live Fluorescensmikroskopi til Monitor funksjonelle egenskaper nyisolerte PKD Epitel

DOI:
10.3791/53035-v

•

08:46 min

•

September 01, 2015

•

, , , , ,
1Department of Physiology,Medical College of Wisconsin, 2Department of Integrative Biology & Pharmacology,University of Texas Health Science Center at Houston

Chapters

  • 00:05Title
  • 02:14Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments
  • 05:02Single Channel Patch-clamp Electrophysiology
  • 05:32Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells
  • 06:55Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats
  • 08:08Conclusion

Summary

자동 번역

자동 번역

Ionekanaler uttrykt i nyretubuli epitel spille en betydelig rolle i patologi polycystisk nyresykdom. Her beskriver vi eksperimentelle protokollene som brukes til å utføre patch-clamp analyse og intracellulær kalsium nivå målinger ved cystisk epitel fersk isolert fra gnager nyrer.

Tags

Keywords: Patch ClampLive Fluorescence MicroscopyPolycystic Kidney DiseasePKDARPKDIon ChannelsCalcium SignalingEpithelial MonolayerEx Vivo AnalysisPCK RatSprague Dawley RatNative SettingEpithelial IsolationImmunostainingPrimary CultureBiochemical Assays

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/53035?article_type=v

Methods for Patch Clamp Capacitance Recordings from the Calyx
check_url/kr/53035?article_type=v

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
check_url/kr/53035?article_type=v

Pressure-polishing Pipettes for Improved Patch-clamp Recording
check_url/kr/53035?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/kr/53035?article_type=v

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
check_url/kr/53035?article_type=v

Fluorescens-basert Måling av Store-opererte Calcium Entry i levende celler: fra Helstøpt Cancer Cell til Skeletal Muscle Fiber
check_url/kr/53035?article_type=v

Kvantitative Live-Cell fluorescens-mikroskopi Analyse av Fission gjær
check_url/kr/53035?article_type=v

Giant Liposome Forberedelse til Imaging and Patch-Clamp Electrophysiology
check_url/kr/53035?article_type=v

En kanal Cell-festet Patch-clamp opptak
check_url/kr/53035?article_type=v

Tilberedning av et trans Protein, den Spenning ionekanal, KvAP, inn Giant unilamellær Blemmer for Mikros og Patch Clamp Studier

Read Article