Journal
/
/
El estudio de la función de proteínas y el Papel de la expresión de la proteína alterada por la interferencia de anticuerpos y reconstrucciones tridimensionales
JoVE 신문
생체공학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생체공학
Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
DOI:

11:57 min

April 21, 2016

, , , ,

Chapters

  • 00:05Title
  • 01:23Preparations
  • 02:36Chariot Complex Formation
  • 03:51Transfecting the Cells
  • 05:41Imaging and Reconstruction
  • 09:35Results: Antibody Interference in HEK293 Cells and Primary Hippocampal Neurons
  • 11:08Conclusion

Summary

자동 번역

Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.

Related Videos

Read Article