Journal
/
의학
/
的肿瘤特异性内皮细胞的分离与培养扩展
JoVE 신문
의학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 의학
Isolation and Culture Expansion of Tumor-specific Endothelial Cells
Please note that all translations are automatically generated. Click here for the English version.

的肿瘤特异性内皮细胞的分离与培养扩展

DOI:
10.3791/53072-v

•

10:15 min

•

October 14, 2015

•

, ,
1Department of Cell Biology & Physiology,University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 3McAllister Heart Institute, University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 01:33Isolating Tumors from Mice
  • 02:28Tumor Disscociation and Immunomagnetic Separation
  • 06:46Endothelial Cell (EC) Colony Selection Using Rings
  • 08:31Results: Isolation of Pure Tumor Endothelial Cell Populations
  • 09:47Conclusion

Summary

자동 번역

자동 번역

We report a reliable method to isolate and culture primary tumor-specific endothelial cells from genetically engineered mouse models.

Tags

Tumor-specific Endothelial CellsTECTumor AngiogenesisEndothelial-to-mesenchymal TransitionImmunomagnetic EnrichmentColony SelectionIn Vitro ExpansionCdh5cre:ZsGreenl/s/l Reporter Mice

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

人牙髓干细胞的分离，鉴定和比较性恒牙使用两种不同的方法，得出

来自患者的细胞培养和分离的CD133<sup> +</sup>假定的肿瘤干细胞的恶性黑色素瘤

分离，培养和成像胎儿胰腺细胞集群

分离，冻存和文化的人羊膜上皮细胞的临床应用

人淋巴管内皮细胞中的多参​​数荧光激活细胞分选分离

分离培养犬从动脉和静脉主血管内皮细胞

从健康志愿者和它们的迁徙可能通过血清中的影响心脏手术后的内皮祖细胞的分离

制备和<em>体外</em>磁化的表征的miR-改性内皮细胞

3D 细胞培养和非抗凝全血对内皮细胞抗凝及抗炎作用的评价

肌肉失衡: 运动人群功能性偏心肌筋强度的检测与训练

Read Article