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JoVE Journal Developmental Biology
Mittels konfokaler Analyse
 
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Mittels konfokaler Analyse

Article DOI: 10.3791/53162-v • 08:10 min • December 14th, 2015
December 14th, 2015 •
1, 2, 3, 4, 5, 5
1Department of Biomedical Science, The Bateson Centre, University of Sheffield, 2Institute of Genetic Medicine, Newcastle University, 3Department of Cardiovascular Science, The Bateson Centre, University of Sheffield, 4School of Biochemistry, University of Bristol, 5Biology Department, University of York

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Das Manuskript hier bietet eine einfache Reihe von Methoden für die Sekretion und Verbreitung von fluoreszierend markierten Liganden in Xenopus-Analyse. Dies bietet einen Rahmen für die Prüfung der Fähigkeit von anderen Proteinen zu Ligand Verteilung ändern und erlaubt Experimente, die Einblick in die Mechanismen, die Morphogen Gradienten geben kann.

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Entwicklungsbiologie Heft 106 Igel flügellose GFP RFP Entwicklungsbiologie Strukturierung sezerniert Liganden Zellsignalisierung
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