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Chapters
- 00:05Title
- 01:32Dehydration and Pretreatment
- 03:32Hybridization and Amplification
- 05:35Signal Detection
- 06:20Image Particle Analysis
- 07:31Results: A Light Microscopic View of Hybridization Signals and the Effect on Hypothalamic htr2a Expression in Rats Treated with MDMA
- 08:16Conclusion
This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.
