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Identification des bactéries pathogènes rares par 16S ARNr Gene Séquençage et MALDI-TOF MS
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
DOI:

06:34 min

July 11, 2016

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Chapters

  • 00:05Title
  • 01:12Preparation of Bacteria for MALDI-TOF MS Analysis
  • 02:05Performing the MALDI-TOF MS Analysis
  • 04:18Results: MALDI-TOF Spectra and Assigned Scores of Rare Pathogens
  • 05:03Conclusion

Summary

자동 번역

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and molecular techniques (16S rRNA gene sequencing) permit the identification of rare bacterial pathogens in routine diagnostics. The goal of this protocol lies in the combination of both techniques which leads to more accurate and reliable data.

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