This content is Free Access.
Chapters
- 00:05Title
- 00:39Preparation of Media and Cultures
- 01:58Growth Phase
- 03:19Ministring Production and Harvesting
- 04:31Results: Double-stranded Ministring Production
- 05:13Conclusion
The linear covalently closed (LCC) DNA minivector (DNA ministring) is a non-viral gene delivery vector offering high transfection efficiency and is relevant to any DNA delivery application. The production system is simple, rapid, and scalable in vivo. The following protocol provides visual step-by-step instructions for the production of DNA ministrings.
