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Chapters
- 00:05Title
- 01:59In Vivo SERCaMP Assay – AAV-GLuc-SERCaMP Injection
- 04:05In Vivo SERCaMP Assay – Blood Sample Collection
- 05:45Gaussia Luciferace Assay
- 06:39Results: Endoplasm Calcium Homeostasis Assessment Using GLuc-SERCaMP
- 08:07Conclusion
Endoplasmic reticulum calcium homeostasis is disrupted in diverse pathologies. A secreted ER calcium monitoring protein (SERCaMP) reporter can be used to detect disruptions in the ER calcium store. This protocol describes the use of a Gaussia luciferase SERCaMP to examine ER calcium homeostasis in vitro and in vivo.
