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在体外产生的常见淋巴祖使用AC-6馈线系统的小鼠浆细胞样树突细胞
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
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在体外产生的常见淋巴祖使用AC-6馈线系统的小鼠浆细胞样树突细胞

DOI:
10.3791/53211-v

•

08:18 min

•

November 23, 2015

•

, ,
1Graduate Institute of Immunology,National Taiwan University College of Medicine, 2Department of Biochemistry,Chang Gung University College of Medicine, 3Molecular Medicine Research Center,Chang Gung University College of Medicine

Chapters

  • 00:05Title
  • 01:03Bone Marrow Cell Isolation
  • 03:25Common Lymphoid Progenitor (CLP) Co-culture and Flow Cytometric Analysis
  • 06:01Results: Representative Murine CLP-derived pDC
  • 07:28Conclusion

Summary

자동 번역

자동 번역

In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.

Tags

Plasmacytoid Dendritic CellsPDCsCommon Lymphoid ProgenitorsCLPsAC-6 Feeder SystemIn Vitro GenerationType I InterferonIFN-ICDCs

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