JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:41DNA Extraction from Tumor Samples
- 04:00DNA Quality Assessment
- 05:33VDJ PCR
- 07:30VDJ Amplicon Library Preparation
- 12:32Results: VDJ-sequencing Traces Clonal Evolution Patterns of B Cell Lymphoma Relapse
- 14:27Conclusion
This protocol describes an approach to interrogate the recombined immunoglobulin heavy chain VDJ regions of lymphomas by deep-sequencing and retrieve VDJ rearrangement and somatic hypermutation status to delineate clonal architecture of individual tumor. Comparing clonal architecture between paired diagnosis and relapse samples reveals lymphoma relapse clonal evolution modes.
