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Quantification of Colonic Stem Cell Mutations
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Quantification of Colonic Stem Cell Mutations

Quantification of Colonic Stem Cell Mutations

DOI:
10.3791/53240-v

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07:53 min

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September 25, 2015

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,
1Department of Pharmaceutical Sciences,University of Pittsburgh

Chapters

  • 00:05Title
  • 02:10Preparation of Glucose-6-phosphate Dehydrogenase (G6PD) Staining Mixture
  • 03:56Tissue Section Staining
  • 05:19Determination of Mutation Frequency
  • 06:14Results: Colonic Stem Cell Mutation Frequency as Determined by Analysis of G6PD Mutant Crypts
  • 07:12Conclusion

Summary

자동 번역

자동 번역

We report significant improvements for the reproducible measurement of somatic colonic stem cell mutations after exposure of mice to potential DNA damaging agents.

Tags

Keywords: Stem Cell MutationsGlucose-6-phosphate Dehydrogenase GeneG6PD EnzymeOptimal Cutting Temperature Compound (OCT)CryptMutation FrequencyEnzymatic Assay

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