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Chapters
- 00:05Title
- 00:46In Vivo Membrane-targeted Expression
- 03:09Isolation from Membrane Fractions
- 04:41Purification Using Immobilized Metal-affinity Chromatography (IMAC), Ion-exchange, and Gel Filtration
- 07:09Heat Modifiability Assay
- 08:35Results: Crystals to Structure Determination for the Putative Vaccine Target YiuR from Yersinia pestis
- 09:29Conclusion
β-barrel outer membrane proteins (OMPs) serve many functions within the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Here, we hope to alleviate a known bottleneck in structural studies by presenting protocols for the production of β-barrel OMPs in sufficient quantities for structure determination by X-ray crystallography or NMR spectroscopy.
