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Chapters
- 00:05Title
- 00:39Cell Culture and Generation of Aggregates
- 03:12Stimulation of Cells in Aggregates
- 04:29Cell Aggregate Fixation, Permeabilization and Blocking
- 07:05Antibody Binding and Detection
- 09:53Results: Morphological Evaluations of mESC Aggregate Culture
- 10:57Conclusion
We have developed a protocol to generate aggregates of mouse embryonic stem cells that display self-organization, symmetry breaking and elongation paralleling axial development. This technique allows the study of axial developmental processes and the generation of cell types that are otherwise difficult to perform in monolayer culture.
