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Journal / Immunology and Infection / An Optimized Method for Isolating and Expanding…
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Immunology and Infection

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JoVE Journal Immunology and Infection
An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
 

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

Article DOI: 10.3791/53256-v • 09:01 min • October 29th, 2015
October 29th, 2015 •
1,2, 1,2, 1,2
1Department of Rheumatology, Laboratory for Molecular Immunology and Inflammation, Ghent University Hospital, 2VIB Inflammation Research Center, Ghent University

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요약
Automatic Translation

Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.

Tags

Invariant Natural Killer T Cells INKT Cells Mouse Spleen Cell Isolation Cell Expansion CD1d Tetramer FACS In Vitro Culture Recombinant Cytokines TCR Stimulation IL-7
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