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Chapters
- 00:05Title
- 01:57Isolation of Fetal Testes from Mus musculus
- 04:45Culturing of Gonad with a Small Molecule Inhibitor
- 07:01Results: Ex Vivo Droplet Culture of Fetal Whole Gonad-mesonephros Complexes
- 08:32Conclusion
The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.
