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Détection de intracellulaire Gene Expression dans des cellules vivantes de murin, humain et porcin Origine utilisant des nanoparticules marquées par fluorescence
JoVE 신문
생물학
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JoVE 신문 생물학
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
DOI:

08:14 min

November 13, 2015

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Chapters

  • 00:05Title
  • 01:41Cell Culture Treatment with Nanoparticles
  • 03:42Identification of Cy3-positive Cells via Flow Cytometry
  • 05:53Results: Detection of Pluripotency Marker Gene Expression in Live iPSCs and Embryonic Stem Cells
  • 07:36Conclusion

Summary

자동 번역

This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.

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