Estable y eficiente modificación genética de células en el ratón adulto V-SVZ para el análisis de neural de células madre Autónoma y los efectos no autónomos
Here we describe a procedure based on the use of lentiviral particles for the long-term genetic modification of neural stem cells and/or their adjacent ependymal cells in the adult ventricular-subventricular neurogenic niche which allows the separate analysis of cell autonomous and non-autonomous, niche-dependent effects on neural stem cells.