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Chapters
- 00:05Title
- 01:00Induction of GL261 Firefly Luciferase Reporter Expression
- 02:31In Vitro Proliferation Assay
- 03:40Tumor Cell Implantation
- 06:16Bioluminescence Imaging of GL261 Tumor Growth
- 07:26Results: Representative In Vitro and In Vivo GL261 Luciferase Expression
- 08:31Conclusion
GL261 glioma cells provide a useful immunocompetent animal model of glioblastoma. The goals of this protocol are to demonstrate proper techniques for monitoring intracranial tumor growth using in vivo bioluminescence imaging, and to verify the utility of luciferase-modified GL261 cells for studying tumor immunology and immunotherapeutic approaches for treating glioblastoma.
