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Isolierung der viralen Replikation Compartment angereicherte Teil nuklearen Fraktionen von Adenovirus-infizierten normalen menschlichen Zellen
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
DOI:

10:22 min

November 12, 2015

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Chapters

  • 00:05Title
  • 00:57Preparation of the Subnuclear Fraction Enriched in Adenoviral Replication Compartments
  • 05:54Immunofluorescence Visualization of RCf
  • 08:00Results: Presence of Adenoviral Components in the Replication Compartment Fraction from Infected Human Foreskin Fibroblasts
  • 08:49Conclusion

Summary

자동 번역

We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.

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