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Chapters
- 00:05Title
- 01:02Data Mining for Transcription Factor Binding Sites in the Promoter Regions of MicroRNA
- 02:20Producing a Lentivirus-containing shRNA in 293 Human Embryonic Kidney Cell Line
- 04:03Transfection of a Cell with Lentivirus-containing shRNA
- 05:48Results: shRNA Down-regulation of STAT3 MicroRNA Leads to Significant Expression Changes in CLL Cells
- 06:30Conclusion
Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.
