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30日猪胚胎的准确和无酚DNA性别鉴定的PCR

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Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR

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30日猪胚胎的准确和无酚DNA性别鉴定的PCR

DOI:

10.3791/53301-v

•

10:16 min

•

February 14, 2016

•

Milena S. Blanes*¹, Stephen C.M. Tsoi*¹, Michael K. Dyck

¹Department of Agricultural, Food and Nutritional Science,University of Alberta

Chapters

00:05Title
01:12Grinding Embryos
02:35Genomic DNA Preparation Using a Modified Sodium Hydroxide Method
04:40Design Sex-specific PCR Primers and Genomic DNA PCR
07:34Results: Porcine Embryo Sex Determination
08:54Conclusion

Summary

자동 번역

This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.

Tags

DNA Sexing PCR Porcine Embryos Phenol-free Reproductive Physiology Sex Ratio DNA Purification Embryo Samples Liquid Nitrogen Sodium Hydroxide DNA Lysate

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