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Chapters
- 00:05Title
- 00:58Cell Extract Preparation
- 02:47Determination of the Protein Concentration of the Cell Extract Using Protein Estimation Kit
- 03:46Enzymatic Assay
- 05:14Lipid Extraction
- 07:04Results: PEMT Activity is Specific and can be Measured from Whole Cell Extracts without the Need to Purify the Enzyme
- 08:51Conclusion
The present report describes an in vitro enzymatic assay to measure phosphatidylethanolamine methyltransferase activity using Leishmania cell extracts. This assay is based on the transfer of a radioactive methyl group from S-[Methyl-3H]adenosyl-L-methionine onto endogenous phosphatidylethanolamine.
