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Beeldvorming van neutrofielen en monocyten in Mesenteriale Veins door Intravitale Microscopie op verdoofd muizen in Real Time
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JoVE 신문 면역학 및 감염병학
Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
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Beeldvorming van neutrofielen en monocyten in Mesenteriale Veins door Intravitale Microscopie op verdoofd muizen in Real Time

DOI:
10.3791/53314-v

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09:28 min

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November 16, 2015

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, ,
1Department of Pathology and Immunology,University of Geneva

Chapters

  • 00:05Title
  • 01:00Bone Marrow Single Cell Suspension Preparation
  • 02:38Neutrophil Enrichment
  • 04:18Neutrophil Labeling
  • 05:29In vivo Measurement of Neutrophil and Monocyte Behavior
  • 07:44Results: Representative Intravital Neutrophil and Monocyte Imaging
  • 08:46Conclusion

Summary

자동 번역

자동 번역

We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

Tags

Keywords: Intravital MicroscopyLeukocyte MigrationMonocytesNeutrophilsCX3CR1gfp/wt MousePam3SK4Vascular InflammationLeukocyte Transendothelial MigrationReal-time Imaging

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