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Chapters
- 00:05Title
- 01:22Short and Long-term Culture of Rat Hippocampal Neurons
- 04:59Fluorescence Imaging of Cytosolic Ca2+ Concentration
- 07:23Biolumninescence Imaging of Mitochondria Ca2+ Concentration
- 11:17Results: NMDA Increases Cytosolic [Ca2+] and Induces Mitochondrial Ca2+ Overload in Hippocampal Neurons
- 12:52Conclusion
Intracellular Ca2+ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca2+ overload. We aimed at investigating Ca2+ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca2+ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.
