Journal
/
/
多功能,微管为基础的法团细菌机械敏感离子通道与刺激的液滴界面双层膜
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
DOI:

09:54 min

November 19, 2015

, , , , , ,

Chapters

  • 00:05Title
  • 00:48Preparation of Liposomes
  • 02:00Manufacture of the Oil Reservoir and Preparation of Electrodes
  • 04:06Setting Up the Experiment
  • 06:22Formation of the Lipid Bilayer
  • 07:49Results: Bilayer Formation and Activation of MscL Channels in Response to Harmonic Mechanical Stimuli
  • 08:59Conclusion

Summary

자동 번역

细菌机械敏感性通道可以用作生物分子器件mechanoelectrical换能器。液滴界面双层(的DIB),细胞启发积木这样的设备,代表新的平台,其中包含与刺激机械敏感通道。在这里,我们表现出成形的DIB,允许在机械刺激机械敏感性通道的研究的一个新的微管为基础的方法。

Related Videos

Read Article